Practical performance of hippocampal tissue resection in rats in pharmacomolecular research

Abstract

AIM: To explain how to resect hippocampal tissue in rats used as live mammalian subjects for the resection of fresh cerebral tissue in laboratories.MATERIAL and METHODS: Adult male Wistar-Albino rats (n=50) were used for this purpose.RESULTS: The average bodyweight of the rats was 316.4 +/- 11.71 g, and the average weight of the resected hippocampal tissues was 1.01 +/- 0.03 g; however, there were no statistically significant differences between the body weights and the hippocampal tissue weights (p>0.05). The hippocampal tissues to be used in the study were excised practically by preserving the original anatomical configuration without injury to the tissue.CONCLUSION: This paper elucidates a simple, step-by-step methodology for performance in the laboratory in order to improve the standardization of hippocampal tissue dissection.