The importance of antioxidant status in gastric intestinal metaplasia

Abstract

Background: Oxidative stress status in different cancer types was investigated before, but not studied in gastric intestinal metaplasia to the best of our knowledge. Purpose of this study is to examine whether there is a difference between oxidative stress status in patients with intestinal metaplasia (IM) compared to individuals without IM, we compared the serum levels of disulfide (SS), total thiol (TT) and native thiol (NT). Methods: This was a prospective, non-randomized casecontrol study including 67 patients with histopathologically confirmed IM and 60 individuals demographically matched in terms of age, gender, BMI, smoking status, and chronic diseases as control group. Results: The mean NT, TT and NT to TT (NT/TT) ratios were statistically significantly higher in IM group compared to controls ((351.71 ± 81.9 _mol/L vs. 271.82 ± 54.13 _mol/L, p=0.000), (391.5 ± 92.69 _mol/L vs. 308.59 ± 55.53 _mol/L, p=0.000) and (0.89 ± 0.6 vs. 0.87 ± 0.29, p=0.022), respectively). The mean SS to TT (SS/TT) ratio was significantly lower in IM group than control group (0.050 ± 0.31 vs. 0.060 ± 0.014, P=0.022). Median SS and mean SS/NT ratio was similar in both groups (16.3 (3.3-78) vs. 18.3 (10-32.7), p=0.271 and 0.055 ± 0.041 vs. 0.070 ± 0.019, p=0.068, respectively). In ROC analysis, cut off value of SS/NT for IM was found 0.062, in regression analysis, SS/NT <0.062 was found as an independently prognostic marker for IM (OR, 2.38; 95%CI: 1.168-4.865, P=0.017). Conclusions: SS/NT ratio lower than 0.062 was found as an independently prognostic marker for IM. This ratio could help to distinguish which patients should be followed closely for gastric cancer.Uvod: Status oksidativnog stresa kod razli?itih vrsta karcinoma istra?en je ranije, ali nije prou?avan u intestinalnoj metaplaziji ?eluca prema našem najboljem znanju. Svrha ove studije je da ispita da li postoji razlika izme?u statusa oksidativnog stresa kod pacijenata sa intestinalnom metaplazijom (IM) u pore?enju sa osobama bez IM, tako što su upore?eni nivoi serumskog disulfida (SS), ukupnog tiola (TT) i nativnog tiola (NT). Metode: Ovo je bila prospektivna, nerandomizirana studija slu?aja i kontrole, koja je obuhvatala 67 pacijenata sa histo patološki potvr?enim IM i 60 osoba demografski podudarnih u pogledu starosti, pola, BMI-a, statusa pušenja i hroni?nih bolesti kao kontrolna grupa. Rezultati: Srednji odnosi NT, TT i NT prema TT (NT/TT) bili su statisti?ki zna?ajno ve?i u IM grupi u pore?enju sa kontrolama (351,71 ± 81,9 mol/L naspram 271,82 ± 54,13 mol/L, p=0,000), (391,5 ± 92,69 mol/L na - suprot 308,59 ± 55,53 mol/L, p=0,000) i (0,89 ± 0,6 naspram 0,87 ± 0,29, p=0,022), respektivno. Srednji odnos SS prema TT (SS/TT) bio je zna?ajno ni?i u IM grupi od kontrolne grupe (0,050 ± 0,31 naspram 0,060 ± 0,014, P=0,022). Medijana SS i srednji odnos SS/NT bili su sli?ni u obe grupe 16,3 (3,3-78) naspram 18,3 (10-32,7), p=0,271 i 0,055 ± 0,041 naspram 0,070 ± 0,019, p=0,068, respektivno). U ROC analizi utvr?ena je grani?na vrednost SS/NT za IM 0,062, u regresionoj analizi SS/NT <0,062 kao nezavisno prognosti?ki marker za IM (OR, 2,38; 95% CI: 1,168-4,865, P=0,017). Zaklju?ak: SS/NT odnos ni?i od 0,062 prona?en je kao nezavisno prognosti?ki marker za IM. Ovaj odnos bi mogao da pomogne da se dijagnostikuju pacijenti kod kojih treba pa?ljivo pratiti rak ?eluca.