Oxidative stress markers are increased in non-alcoholic fatty liver disease patients with high serum alanine aminotransferase levels

Abstract

Introduction: Non- alcoholic fatty liver disease (NAFLD) is a pathological disease that causes chronic damage in the liver without alcohol consumption. Aim of the research: To determine oxidative stress and inflammation statuses (NAFLD) with increased alanine aminotransferase (ALT). Material and methods: Oxidative stress (malondialdehyde (MDA), advanced oxidation protein products (AOPP), inflammation (serum tumour necrosis factor-alpha (TNF-alpha)), interleukin-6 (IL-6), and hyaluronic acid (HA) were measured by the ELISA method or manually. A total of 133 non-obese and non-diabetic individuals were included. Patients diagnosed with NAFLD with normal ALT levels were included in group 1 (n = 53), and those with high ALT levels were included in group 2 (n = 35). The control group included individuals without any known systemic disease (n = 45). Results: We found that the serum MDA levels were significantly elevated in group 2 in comparison to the control group (p < 0.05). However, there were no significant changes in the serum AOPP levels in any group (p > 0.05). The serum TNF-a and IL-6 levels of group 1 and group 2 were higher than those in the control group (p < 0.05). The serum HA levels in group 2 increased significantly in comparison to the other groups (p < 0.05). Conclusions: HA and MDA were enhanced with high ALT levels and with elevated TNF-alpha and IL-6 levels without high ALT levels in NAFLD.Wprowadzenie: Niealkoholowa st?uszczeniowa choroba w?troby (NAFLD) jest schorzeniem wywo?uj?cym przewlek?e uszkodzenie w?troby, którego przyczyn? s? inne czynniki ni? nadmierne spo?ycie alkoholu. Cel pracy: Oznaczenie stresu oksydacyjnego i wyst?powania stanu zapalnego u pacjentów z NAFLD z podwy?szon? aktywno?ci? aminotransferazy alaninowej (ALT). Materia? i metody: Markery stresu oksydacyjnego (aldehyd dimalonowy (MDA) i zaawansowane produkty utleniania bia?ek (AOPP)) oraz stanu zapalnego (czynnik martwicy nowotworów ? (TNF-?)), interleukina-6 (IL-6) i kwas hialuronowy (HA)) w surowicy oznaczano testem ELISA lub metod? manualn?. Do badania w??czono ogó?em 133 osoby bez nadwagi i cukrzycy. Pacjentów z rozpoznaniem NAFLD, u których st??enie ALT by?o prawid?owe, w??czono do grupy 1. (n = 53), natomiast chorych z NAFLD i wysokim st??eniem ALT – do grupy 2. (n = 35). Grupa kontrolna obejmowa?a osoby, u których nie wyst?powa?y ?adne znane choroby ogólnoustrojowe (n = 45). Wyniki: Stwierdzono, ?e st??enia MDA w surowicy w grupie 2. s? istotnie podwy?szone w porównaniu z grup? kontroln? (p < 0,05). Nie odnotowano natomiast istotnych zmian w st??eniach AOPP w surowicy w ?adnej z grup (p > 0,05). St??enia TNF-? i IL-6 w surowicy w grupie 1. i 2. by?y wy?sze ni? w grupie kontrolnej (p < 0,05). St??enie HA w surowicy w grupie 2. by?o istotnie podwy?szone w stosunku do pozosta?ych grup (p < 0,05). Wnioski: W badaniu wykazano podwy?szone st??enia HA i MDA u pacjentów z NAFLD z wysokim st??eniem ALT oraz podwy?szone st??enia TNF-? i IL-6 u osób z NAFLD z prawid?owym st??eniem ALT.