Evaluation of perilipin expression in centrifuged fat grafts on different revolutions per minute and duration combinations

Abstract

Fat graft applications are one of the most frequently used procedures in reconstructive and aesthetic surgery. Fat graft survival rate is variable and depends on many parameters. Those are the features of recipient area, method of obtaining the fat graft, the procedures applied to the harvest to get pure fat graft and the application techniques. In this study, we aimed to find the optimal revolutions per minute(rpm) and duration to be used in the centrifugation technique, which is one of the most frequently used methods in fat grafting. In our study, we used perilipin protein as a survival marker. Liposuction samples were obtained from 10 Caucasian women individuals; whose age range is between 22 and 29 years without any other reported health problem. Fat grafts from all patients were taken by the same surgical team by the same liposuction procedure. The grafts obtained in our study were divided into 6 different groups, these are A1 group (1 minute–1000 rpm) A2 group (3 minutes–1000 rpm) A3 group (5 minutes–1000 rpm) B1 group (1 minute–3000 rpm) B2 group (3 minutes–3000 rpm) and B3 group (5 minutes–3000 rpm). Perilipin expression measurements were made in the sections taken by immunohistochemistry technique. Three minutes–3000 rpm combination (B2 group) was found to be perilipin stained statistically significantly higher than other combinations. This study seeks to make a notable contribution to the literature by demonstrating the relation between frequently used centrifuge combinations and measuring the expression of perilipin. This protein’s role in adipocyte regulation is becoming increasingly apparent. Level of Evidence III This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.